To start cleaning and isolating on agar, I recommend putting all your spores and clones on agar first, by doing so, you can ensure that you are working with a clean culture before you introduce it to grains, and it will also take out some the guess work of spore viability.
Once you have a culture growing on agar, you will now want to make sure that it is clean and free of contaminates.
To demonstrate how to clean up cultures when they need it, please see below 3 images of a few clone cultures with varying levels of growth and infections.
On “image A” we have some mycelial growth in amongst multiple different contamination points. If you had multiple plates that you had cloned from the same mushroom you may want to discard this and choose a cleaner plate. If this is all you have to work with it is best to decide where to take your transfer from before you start.
| With “image B” you can see it is mostly clean with only a small area of contamination also around the 4:30 mark, most other areas are fine for a transfer. |
“Image C” looks contamination free and ready for use but it is always a good idea to do 2 or 3 transfers from your initial clone to make sure you have a clean plate
Liquid Culture Starter Pack
- 2 x syringes
- 2 x needles
- 2 x MEA plates
- 2 x pieces of parafilm
- 2 x Swabs
This is enough to make 1lt of liquid Culture.
To correctly use this product you should be working with at least a Still Air Box or Laminar Flow Hood. You will need a modified jar to grow this culture in, if you dont have one you can purchase ours from here. Step-bu-step information can be found below.
Premade MEA Agar Plate
MyShrooms malt extract agar plates are made with the perfect blend of agar, malt extract, & yeast extract. They are pressure sterilized and poured in our lab using laminar flow hood.
PLEASE NOTE this product is made to order and may delay your delivery time.
Packs of 10 x 90mm
Leave a Reply